This product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Eraser, the sample processed by gDNA Eraser can be directly used for reverse transcription reaction to synthesize cDNA. This kit is equipped with the novel and efficient reverse transcriptase, HiFiScript, featuring significantly improved transcription activity due to innovative mutation sites. The efficiency and yield of the first-strand cDNA synthesis are enhanced, allowing the synthesis of the first strand with pg-level total RNA or mRNA. If the reverse transcription product, is used for downstream fluorescence quantitative detection, the reverse transcription reaction can be completed at 42 °C for 15 minutes. This kit is suitable for first-strand cDNA synthesis and subsequent applications such as RT-PCR, RT-qPCR, and preparation of full-length cDNA libraries.