This kit is specially developed for one-step RT-PCR experiments. Reverse transcription and PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cover during the reaction process, which improves the detection sensitivity and experimental efficiency while avoiding contamination. The kit includes a new high-efficiency reverse transcriptase, a rapid hot-start DNA polymerase, reaction buffers for reverse transcription and PCR amplification, and other components necessary for your experiment. SuperRT V reverse transcriptase lacks RNase H activity, reducing RNA degradation in reverse transcription reactions. This reverse enzyme has a high reverse transcription efficiency and can perform a good reverse transcription reaction on a small amount of RNA template. The rapid hot-start DNA polymerase used in the PCR reaction has excellent performance of high amplification efficiency, strong specificity, and fast extension speed. The unique buffer system allows for maximum efficiency of reverse transcriptase and polymerase. The target product amplified using this kit has an "A" base attached to the 3′ end and can be used directly for T/A cloning.