2×GoldStar MasterMix is a premix system composed of GoldStar DNA Polymerase, Mg²⁺, dNTPs, PCR stabilizer and reinforcer. It has the advantages of simple and rapid operation, and can minimize human error and contamination. The GoldStar DNA Polymerase included in the product is a chemically modified Taq DNA polymerase. The chemically modified enzyme has no polymerase activity at room temperature and is effective in avoiding the nonspecific amplification caused by the nonspecific binding of primer and template or primer dimer at room temperature. The activation of the enzyme requires incubation at 95 ℃ for 10 min. The unique buffer system makes the enzyme suitable for more application, so that the template with high GC content, complex secondary structure and low copy can be efficiently amplified, and the unique MasterMix formula makes the whole reaction system more stable. This product has been added dye (blue), and can be directly electrophoresis detected after the reaction. Most PCR products obtained by amplification have an "A" base attached to the 3 'end, so they can be directly used for T/A cloning. This product has strong specificity, and does not require purification to remove impurity after PCR amplification, and can be directly used in downstream cloning or chip hybridization experiments. It is suitable for conventional PCR, RT-PCR and multiplex PCR experiments, and is especially suitable for PCR reactions with high specificity requirements.