This kit provides the adapter and primer for NGS library preparation. It is designed for Illumina platform, and index primer is designed to meet the need to prepare multiple library samples for multiple high-throughput sequencing. The library prepared can be used for the sequencing of multiple platforms such as Illumina GAIIx, HiSacnSQ, HiSeq 2500/2000/1000 and MiSeq sequencing, etc.