This product is a dedicated kit for one-step Real-Time RT-qPCR using the probe method (TaqMan, Molecular Beacon, etc.). When using this product for Real-Time RT-qPCR reactions, reverse transcription and quantitative PCR are performed in the same reaction system, and there is no need to add reagents or open the cap during the reaction, which avoids contamination and improves experimental efficiency. This product has high detection sensitivity, strong fluorescence signal and high signal-to-noise ratio, which is very suitable for the detection of trace RNA such as RNA viruses. It contains a special buffer system that maximizes the efficiency of reverse transcriptase and DNA polymerase. The use of this product can obtain a wider linear range, more accurate quantification of the target gene, good reproducibility, and high reliability. The dUTP/UNG anti-contamination system is introduced into this kit, which rapidly degrades U-containing contaminants at room temperature.