This product is a one-step Real-Time RT-qPCR kit. The included SYBR Green I
fluorescent dye binds to all double-stranded DNA, allowing the product to be used for
the detection of a wide range of different target sequences without the need to
synthesize specific labeled probes. Real Time RT-qPCR reactions, reverse
transcription and quantitative PCR are performed in the same reaction system, and
there is no need to add reagents or open the caps during the reaction, which avoids
contamination and improves experimental efficiency. The new high-potency reverse
transcriptase lacks RNase H activity, reducing RNA degradation in the reverse
transcription reaction. The enzyme has a high reverse transcription efficiency and can
perform a good reverse transcription reaction on a small amount of RNA template. It
has high affinity with RNA, and can read through RNA templates with high GC content
and complex secondary structure. The new high-efficiency hot-start enzyme blocks the
activity of the enzyme at room temperature, thus effectively avoiding the non-specific
amplification caused by the non-specific binding of primers and templates or primer
dimers at room temperature, which greatly improves the accuracy of the real-time PCR
reaction. The included buffer system maximizes the efficiency of both enzymes. This
product has high sensitivity, high specificity, and a wide linear range, which makes the
quantification of the target gene more accurate.
Catalog number |
Specifications |
CW2624S |
100 rxns |