The kit is a specially developed kit for the Illumina high-throughput sequencing platform, which provides the enzyme master mix and reaction buffer required for genomic DNA library preparation, and contains all components except PCR primers. Compared with the traditional library preparation kit, this kit uses a new transposase method for library preparation. It can complete the DNA fragmentation, end repair and adapter ligation reaction through a simple enzymatic reaction, which significantly reduces the amount of template used, reduces the experimental operation steps, and shortens the library preparation time. This kit uses high-fidelity DNA polymerase for library enrichment, performs PCR amplification without preference, and expands the coverage area of the sequence, which can effectively prepare DNA libraries for Illumina second-generation sequencing platform.The kit is suitable for starting template DNA inputs of 5 ng. All reagents in the kit have undergone strict quality control and functional validation to ensure maximum stability and reproducibility of library preparation.