The single-cell whole-genome amplification kit is based on the isothermal amplification system of MDA, which can achieve whole-genome amplification with a single cell or micro sample as a template. After the whole genome of a single cell is amplified, the size of the amplified product is 2-100 kb. It can be widely used in next-generation sequencing, large fragment CNV analysis, microsatellite analysis, qPCR analysis, gene chip analysis, etc. The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from bacteriophage. It has strong strand displacement activity and chain affinity. A single polymerization reaction can achieve continuous polymerization extension of up to 100 kb, and its amplified product is suitable for a variety of downstream applications. Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, ensuring high fidelity in DNA synthesis. A single reaction normally yields greater than 20 μg of genomic DNA with high coverage.