The product is a premix system consisting of Es Taq DNA Polymerase, Mg²⁺, dNTPs, PCR stabilizers and enhancers at a concentration of 2×. Es Taq DNA Polymerase has high amplification efficiency and low mismatch rate. The original MasterMix formula makes the whole reaction system very stable, over 98% success rate in PCR amplification on the first attempt, while complex templates can be effectively amplified, and can minimize human error and contamination. This product does not contain dye, after the PCR procedure is completed, appropriate amount of loading buffer can be added according to the need for electrophoresis. Most PCR products obtained by amplification have an "A" base attached to the 3 'end, so they can be directly used for T/A cloning. It is mainly suitable for conventional PCR reaction and high fidelity gene cloning and other experiments.