Lyophilized WinScript Universal One Step RT-qPCR Mixture is a specialized lyophilized reagent for quantitative PCR reactions using RNA as a template. It includes a novel Taq DNA polymerase modified with antibodies, highly efficient heat-stable reverse transcriptase, enhancers, and stabilizers. The buffer contains Mg2+ and dNTP. Additionally, it incorporates factors that effectively suppress nonspecific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This reagent enables efficient primer amplification while facilitating multiplex fluorescent quantitative amplification reactions. It is user-friendly, requiring only the addition of primers and probes, and the nucleic acid samples can be directly used for amplification after extraction. The product is compatible with both single-plex and multiplex probe-based qPCR reaction systems, suitable for conventional and rapid detection.