Lyophilized Animal Detection Probe Mixture (UNG) is a dedicated in situ full-component lyophilized reagent for probe-based detection of DNA viruses, including new antibody-modified Taq DNA polymerases, PCR buffers, dNTPs, Mg2+, enhancers and stabilizers. It is only needed to add the extracted template, primers, probes, which is easy to use. The product is compatible with both single and multiplex probe method qPCR reactions.

The dUTP-UNG anti-contamination system is used in this product. dUTP is added during the preparation of the PCR reaction system, therefore form the amplification product containing dU bases. This product can be eliminated by the UNG enzyme treatment in the PCR system before the next PCR reaction. This effectively removes residual contamination of the PCR product and greatly reduces false positives due to contamination of the amplification product. The UNG enzyme is inactivated by the pre-denaturing step in the PCR cycle, so it does not affect the formation of new dU-based PCR products.