GoldHi EndoFree Plasmid Maxi Kit (Plus)

Endotoxin is a common pollutant in plasmid extraction. Eukaryotic cells are very sensitive to endotoxin, so if the plasmid contains endotoxin, it will greatly reduce the transfection efficiency of eukaryotic cells. On the basis of alkaline lysis of cells, this kit adopts unique silicon matrix membrane adsorption technology, efficient and specific binding plasmid DNA; and special endotoxin-free Buffer ER and Endo-Remover FQ, which can effectively remove endotoxin, protein and other impurities. At the same time, it is equipped with special indicator CWBlue to ensure the efficient completion of plasmid extraction. The 100-300 mL bacterial liquid could be treated each time, and the purity of the plasmid is high, and the extraction amount is large, and the plasmid DNA of 2 mg transfection grade is obtained.