Cowin NGS Fast DNA Library Prep Set (Ion Torrent) provides the enzyme premix system and reaction buffer for DNA library preparation, including all components except the adapters. The prepared library can be used for sequencing on the Ion torrent PGM and Ion Proton second-generation sequencing platforms. Compared with conventional library preparation methods, this kit combines multiple steps and omits multiple purification steps, thus significantly reduces the minimum requirement for starting template DNA and shortens library preparation time. In addition, this kit uses high-fidelity DNA polymerase for library enrichment with unbiased PCR amplification, expands the coverage area of the sequence, enables efficient preparation of DNA libraries for the Ion torrent next-generation sequencing platform.