This product is a probe-based (TaqMan, Molecular Beacon, etc.) one-step Real-Time RT-qPCR kit using RNA as template. When using this product for Real Time RT-qPCR reaction, reverse transcription and quantitative PCR are performed in the same reaction system. There is no need to add reagents or open the tube lid during the reaction, avoiding contamination and improving experimental efficiency. The dUTP/UNG anti-contamination system is introduced in this reagent, which can rapidly degrade U-containing contaminants at room temperature and avoid affecting the efficiency and sensitivity of RT-qPCR. This product contains lyophilized protective agent and can be used for the preparation of lyophilized reagents.