This product is a one-step Real-Time RT-qPCR kit based on probes. Using this product for RT-qPCR reactions, reverse transcription and quantitative PCR are carried out in the same reaction system. No reagents are added during the reaction, and the tube cover will not be opened, thus avoiding the contamination and improving the experiment efficiency. The sensitivity of this product is high, the fluorescence signal is strong, and the signal-to-noise ratio is high. It is very suitable for the detection of trace RNA such as virus RNA. The included unique buffer system allows the reverse transcription enzyme and DNA polymerase to exert maximum efficiency at the same time to improve efficiency. Using this product can get a wider range of linear, more accurate quantification of targeted gene, high repeatability and high credibility.