HiFi V M-MLV (H-) Reverse Transcriptase is a reverse transcription enzyme in which the mutant M-MLV gene is recombined and expressed by E. coli engineering bacteria. The enzyme can catalyze the polymerization of complementary DNA using RNA or DNA:RNA hybrid chain as template. The mutated HiFi V M-MLV (H-) Reverse Transcriptase lacks RNase H activity, reducing RNA degradation during reverse transcription and making it easier to obtain full-length cDNA. HiFi V M-MLV (H-) Reverse Transcriptase exhibits excellent reverse transcription activity at 55 °C (the enzyme can be used up to 60 °C for reverse transcription reactions), and for RNA with complex structures, increasing the temperature of the reverse transcription reaction can significantly increase the yield of cDNA. In addition, HiFi V M-MLV (H-) Reverse Transcriptase, which is more stable, can synthesize up to 15 kb cDNA. It is suitable for synthesis of first strand cDNA, RT-PCR, RT-qPCR and preparation of full-length cDNA library.