This product can complete the genomic DNA removal and reverse transcription reaction at the same time, which can effectively reduce the risk of sample contamination and RNA degradation caused by complex loading processes. This product is equipped with a new high-efficiency reverse transcriptase, and the new mutation site can greatly improve the transcriptional activity of the enzyme. 5×HiFiScript All-in-one qRT MasterMix is a reverse transcription mastermix, which contains all the reagents required for genomic DNA removal and reverse transcription. It can effectively remove genomic DNA at 50 ℃ for 15 min with the addition of template and water during the reaction, and complete the efficient synthesis of cDNA, which is convenient and fast to operate. The reverse transcription products are compatible with probe method and SYBR method qPCR for efficient gene expression analysis.