This kit provides a simple, fast and efficient extraction method of plasmid, suitable for the extraction of 1-5 mL bacterial fluid, based on the lysis of cells by alkaline lysis method, through the new silicone-based plasmid membrane efficiently and exclusively binding plasmid DNA, each adsorption column can adsorb a maximum of 40 μg of plasmid DNA, and effectively removes the contamination of genomic DNA, RNA, protein and other contaminants, easy and convenient to operate.