2.5 × TFSMaster Mixture is a premix system suitable for various types of multiple PCR at a concentration of 2.5 ×, Contains components such as DNA polymerase, PCR Buffer, dNTPs, Mg2+, and stabilizers and enhancers, making the operation simple and fast. The DNA polymerase contained in TFS Master Mixture is a genetically engineered recombinant enzyme with 5 '→ 3' DNA polymerase activity and 5 '→ 3' exonuclease activity; DNA polymerase is modified by a new type of antibody, which is an antibody modified hot starter enzyme. It can effectively reduce the nonspecific amplification generated by the nonspecific binding of primers and templates or primer dimers under normal temperature conditions. It also has excellent characteristics such as short activation time, strong amplification ability, high sensitivity, and good stability. The combination of a unique PCR buffer system and hot start enzymes significantly improves the amplification efficiency of PCR, with higher sensitivity and stronger inhibitor tolerance. This product has a wide range of applications, not only for general and dye based real-time fluorescence quantitative PCR, but also for forensic multiple STR amplification reactions. It can be used in forensic analysis, paternity testing, scientific research, and other aspects of human genetic identification.
Catalog number |
Specifications |
CW3311S |
1 mL |