Lysozyme

 Lysozyme is an enzyme that destroys bacterial cell walls and improves the efficiency of protein or nucleic acid extraction. By hydrolyzing the β-1,4 glycosidic bond between n-acetyl-muricylic acid and n-acetyl-glucosamine in cell wall, the insoluble mucopolysaccharide is decomposed into soluble glycopeptides, and the contents of the broken cell wall are induced to escape and dissolve bacteria. Gram-positive bacteria (G+) are very sensitive to lysozyme because of their high content of peptidoglycan in their cell walls. Conversely, Gram-negative bacteria (G-) are less sensitive. When EDTA is contained in the system, Ca2+ in the bacterial outer membrane can be chelated and G- sensitivity can be improved. In addition, when lysozyme is used to crack Escherichia coli, the efficiency can be improved significantly when nuclease such as DNase Ⅰ is added. This product is suitable for bacterial cell wall degradation, protoplasmic preparation, bacteriolysis, sample preparation before nucleic acid separation and pharmacological research.