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FastPrime DNA Polymerase is a mixture of Taq monoclonal antibody and Taq DNA Polymerase with high amplification and fidelity. It is suitable for HOT Start PCR. When using FastPrime DNA Polymerase in PCR amplification, the Taq enzyme antibody inhibited the activity of DNA polymerase before denaturation at high temperature, which effectively inhibited the non-specific annealing of primers and the non-specific amplification caused by primer dimers at low temperatures. The Taq enzyme antibody was denatured in the initial DNA denaturation step of PCR reaction, and the DNA polymerase activity gradually restored to achieve the thermal activation effect. This product does not require special inactivation of Taq enzyme antibodies and can be used under conventional PCR reaction conditions. FastPrime DNA Polymerase has 5 '→3' DNA polymerase activity, 5 '→3' exonuclease activity and 3 '→5' exonuclease activity. Compared with Taq DNA Polymerase, FastPrime DNA Polymerase has high amplification efficiency and low mismatch rate, which can efficiently amplify DNA fragments. This product can be used directly for T/A cloning because the 3' end of the amplified PCR product has an "A" base. This product is suitable for routine PCR reaction and gene cloning for high fidelity reaction.
| Catalog number | Specifications |
| CW2977S | 500 U |
| CW2977M | 2500 U |
Nucleic Acid Release Reagent
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