Endotoxin is a common contaminant in plasmid extraction. Because eukaryotic cells are very sensitive to endotoxin, the transfection efficiency will be greatly reduced if endotoxin is contained in the plasmid. This kit provides a simple, rapid and efficient method for the extraction of endotoxin-free plasmids. 100-300 ml of bacterial culture can be processed each time, and up to 2 mg of transfection-grade plasmid DNA can be obtained.Multiple samples can be handled simultaneously by use of a vacuum device and the whole process takes only 45 minutes,which effectively reduces hand-on time
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