NuClean FFPE DNA kit

This kit is suitable for purification of genomic DNA from formalin-fixed, paraffin-embedded tissues(FFPE). The kit uses optimized deparaffinization reagents and lysis buffers to release DNA from formalin-fixed or tissue sectioned samples, is xylene-free, and requires no overnight manipulation. After the digested sample is incubated at a higher temperature, it is cross-linked with formalin that removes free DNA, which effectively improves the yield and purity of DNA; the optimized buffer system enables the DNA in the lysate to be specifically bound to the adsorption membrane. High-purity DNA can be obtained by effectively removing inhibitors through a two-step rinsing step, followed by final elution with low-salt buffer or water. At the same time, it is equipped with a high-efficiency micro-adsorption column, and the elution volume can be as low as 20 μL. The purified DNA can be used directly for PCR, Real-time PCR, SNP genotyping, STR genotyping, next-generation sequencing and pharmacogenomics research.