This product is a one-step Real-Time RT-qPCR kit. The included SYBR Green I fluorescent dye binds to all double-stranded DNA, allowing the product to be used for the detection of a wide range of different target sequences without the need to synthesize specific labeled probes. Real Time RT-qPCR reactions, reverse transcription and quantitative PCR are performed in the same reaction system, and there is no need to add reagents or open the caps during the reaction, which avoids contamination and improves experimental efficiency. The new high-potency reverse transcriptase lacks RNase H activity, reducing RNA degradation in the reverse transcription reaction. The enzyme has a high reverse transcription efficiency and can perform a good reverse transcription reaction on a small amount of RNA template. It has high affinity with RNA, and can read through RNA templates with high GC content and complex secondary structure. The new high-efficiency hot-start enzyme blocks the activity of the enzyme at room temperature, thus effectively avoiding the non-specific amplification caused by the non-specific binding of primers and templates or primer dimers at room temperature, which greatly improves the accuracy of the real-time PCR reaction. The included buffer system maximizes the efficiency of both enzymes. This product has high sensitivity, high specificity, and a wide linear range, which makes the quantification of the target gene more accurate.