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DNase I (RNase Free)
DNase I is a divalent cation-requiring endodeoxyribonuclease that can be used to
degrade single-or double-stranded DNA. The principle is that DNase I hydrolyzes
phosphodiester bonds to generate mononucleotides or oligonucleotides with
5'-phosphate groups and 3'-OH. Either Mg2+ or Mn2+ can activate the activity of DNase
I, while the concentration of Ca2+ directly affects the activity of the enzyme. In the
presence of Mg2+, nicks can be randomly generated on each single strand
ofdouble-stranded DNA; while in the presence of Mn2+ double-stranded DNA can be
broken.
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Storage condition: -20℃.
Days of preservation: 730 days.
Transport conditions: RT.
Application: Mainly used for RNA preparation without DNA contamination, reverse transcription and in vitro transcription experiments.
| Catalog number | Specifications |
| CW2090S | 1000 U |
Nucleic Acid Release Reagent
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