DNase I (RNase Free)

DNase I is a divalent cation-requiring endodeoxyribonuclease that can be used to degrade single-or double-stranded DNA. The principle is that DNase I hydrolyzes phosphodiester bonds to generate mononucleotides or oligonucleotides with 5'-phosphate groups and 3'-OH. Either Mg2+ or Mn2+ can activate the activity of DNase I, while the concentration of Ca2+ directly affects the activity of the enzyme. In the presence of Mg2+, nicks can be randomly generated on each single strand ofdouble-stranded DNA; while in the presence of Mn2+ double-stranded DNA can be broken. Mainly used for RNA preparation without DNA contamination, reverse transcription and in vitro transcription experiments.